FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866526
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55100.038.074961
GGTATCA24050.034.749811
GTGGTAT11100.031.2938961
ATCAACG68400.030.9171033
TCAACGC68550.030.7992884
CAACGCA69000.030.5984255
AACGCAG70000.030.1613046
TATCAAC70250.030.1029132
TGGTATC12000.029.514522
ACGCAGA76450.027.6151497
TATAGCG500.00786225427.508682
CGCAGAG78200.026.9531928
AGAGTAC77350.026.62704811
CAGAGTA77700.026.55135710
GCAGAGT82750.025.4711749
GAGTACG68250.023.82950212
AGTACGG69050.023.60321413
TCGCTAA1400.002809674521.67947670
CTCGTAT1356.6676366E-620.50036839
GTACGGG79950.020.1702314
Produced by FastQC (version 0.11.5)