FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159651
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26500.040.9262351
GGTATCA12050.032.572791
ATCAACG34200.032.3089143
TCAACGC34650.031.9886654
TATCAAC34800.031.7518652
CAACGCA35200.031.391055
GTGGTAT4600.030.6876161
AACGCAG36500.030.367326
CTCGTAT1151.6498234E-930.08966339
CTTATAC1800.028.6916711
TCGTATG1452.1827873E-1128.65444840
ACGCAGA38650.028.5890057
CAGAGTA39950.027.4863710
CGCAGAG40550.027.3343338
ACGAGAC1900.027.17924122
AGAGTAC40250.027.02493711
TGGTATC5850.025.302072
GCAGAGT44550.024.8028059
CGTATCG700.001351427924.58755910
AGTACGG38350.023.06806813
Produced by FastQC (version 0.11.5)