FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684294
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35850.041.8196751
TCAACGC41350.036.0772634
TATCAAC41700.035.6916432
ATCAACG42000.035.4367033
CAACGCA42300.035.2670175
AACGCAG43700.034.058166
GGTATCA15650.033.992761
ACGCAGA47600.031.3392967
CGCAGAG48000.030.9342548
AGAGTAC47700.030.11531311
CAGAGTA50050.028.97728210
GCAGAGT54150.027.6760229
CGTGACG900.00974079426.32504770
AATAGCG3300.025.46977662
AGTACGG38800.024.91946613
GAGTACG38800.024.8304712
TAGCGGC3400.024.76614864
GTGGTAT6200.023.9583471
ATAGCGG3550.023.69753563
TAATCGT3700.022.46066738
Produced by FastQC (version 0.11.5)