FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684294
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.038.65841
GTATCAA37500.037.59941
TATCAAC42650.032.8815542
ATCAACG43250.032.3195843
TCAACGC43450.032.232064
CAACGCA43550.032.077895
AACGCAG45450.031.116376
CGCAGAG48700.028.9689858
ACGCAGA48850.028.8800327
TTAACGG2600.027.92989335
AGAGTAC49100.027.82053811
CAGAGTA51600.026.53791610
GTGGTAT8100.026.452011
GCAGAGT55700.025.3291119
TAACGGC2950.024.62644236
TGGTATC8000.024.1792812
TTTAACG3000.023.04638934
GAGTACG39750.022.82423412
AGTACGG40450.022.42925513
TAACCGT3150.022.13998453
Produced by FastQC (version 0.11.5)