FastQCFastQC Report
Wed 8 May 2024
EGAF00005760908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840503
Total Bases62.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17750.21118306537870773No Hit
GTAATACACGGCCGTGTCCCCGGCTCTCAGGCTGTCCATTTGCAGATACA16370.1947643256478561No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC14920.17751275129297575No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13310.1583575549403155No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13030.15502621644420067No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT11820.14063007508599018No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11210.13337251621945428No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCTACCCTCTCCT11150.13265865797028684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11110.13218275247084188No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10620.12635291010264094No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10270.12218873698249738No Hit
GTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10200.12135590235846869No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10110.12028511498471749No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9930.1181435402372151No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9930.1181435402372151No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT9860.11731070561318639No Hit
GTGTATTACTGTGCGAGAGAGAGGAGGGGGTTCTTGTATGCTTTTGATAT9610.11433629624165528No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9500.1130275561181816No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9440.11231369786901416No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9270.110291099496373No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9220.10969621762206677No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8820.10493716262761704No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8470.1007729895074735No Hit
GAGCTACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCC8430.10029708400802852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)