FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931025
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT19160.2057946886496066No Hit
GTTTTATACAGCTCCACCAATAAGAATTATTTAGCTTGGTACCAGCAGAA18830.20225020810397143No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18380.19741682554174164No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTATTGGTGGAGCTGTATAAAACACT15730.16895357267527725No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13300.14285330683923633No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12340.13254209070647943No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12000.12889020165946136No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11770.126419806127655No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11300.12137160656265943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10920.11729008351010982No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10830.11632340699766386No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10820.11621599849628098No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10740.1153567304852179No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10300.11063075642437098No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAATGAATTCTTGGCGTTGTCTCTGGA10130.10880481190086194No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10050.10794554388979886No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9920.10654923337182137No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9460.10160844230820869No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9350.10042694879299696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17250.039.4209861
GGTATCA7000.037.970211
TGGTATC2950.035.0977752
TATCAAC19700.034.8631942
ATCAACG20000.033.649993
ACGTTAG750.002955714233.59607770
TCAACGC20250.033.234564
GTGGTAT3250.032.9252171
CAACGCA20500.032.8292585
AACGCAG20600.032.6698956
TATACCG450.004613857730.6780536
ATACCGC450.00461749630.6731117
AGAGTAC22550.029.22786911
CAGAGTA23250.028.19947210
CGCAGAG23900.028.1544518
ACGCAGA23950.027.9515917
ATATTAC500.00772017727.6102493
TATTGGT4600.027.18672849
GCAGAGT24600.027.0727639
CTGTATA4950.026.1215760
Produced by FastQC (version 0.11.5)