FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868387
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52800.041.8981931
GGTATCA21050.036.8807451
TCAACGC60900.036.2059334
CAACGCA61350.035.9973075
ATCAACG62950.035.2451023
AACGCAG63700.034.777146
TATCAAC64550.034.3182752
ACGCAGA67950.032.548817
AGAGTAC67200.031.9410711
CGCAGAG69600.031.8265258
GTGGTAT7600.031.6393681
CAGAGTA69350.031.09939210
GCAGAGT72100.030.5800729
GAGTACG61450.027.04896212
AGTACGG61550.027.00501413
TGGTATC9450.025.0772112
CGTATGC2400.024.54970241
ACGGGAT8500.022.62721316
GTACGGG72950.022.59659814
TATACAC3700.022.2777583
Produced by FastQC (version 0.11.5)