FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584192
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50320.31763826606875933No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG34840.2199228376358421No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34320.21664040722336686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33890.21392608976689695No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31090.19625146446895325No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAATGTAAAAT30900.19505211489516422No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30660.19353714701248334No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT30290.19120157152668363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29470.18602543126085727No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28810.18185926958348483No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28160.17775623156789075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27630.17441067749363715No Hit
CACCTGCACTGTGTCTGGTTTCTCCATCACCAGTGGTGGTTACGACTGGA26630.1680983113158001No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25780.16273280006463864No Hit
CCCCTGGAGCGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG25490.1609022138730659No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGAA25150.15875600937260131No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC24970.15761978346059063No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24850.1568622995192502No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23290.14701500828182443No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22680.14316446491334384No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22600.14265947561911688No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22200.14013452914798205No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22140.13975578717731182No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22120.1396295398537551No Hit
GGCCAAAAGTGTACGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA21370.13489526522037731No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21180.1336959156465883No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCC20900.13192845311679394No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAATGTAAAATCTGTGCCTGATCCACTGCCA20700.13066597988122652No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGAAAATCAGCAGAG19290.12176554357047631No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACTCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA19210.12126055427624935No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18700.11804124752555246No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18590.11734688724599038No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18380.11602129034864461No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18260.11526380640730417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17940.11324384923039632No Hit
GCTAATGCTCTGGGTCTCTGGATCCACTGGGGATATTGTGATGACTCAGTCTCCCCTCTCCCTGCCCGTCACCC17430.11002454247969944No Hit
GTGTCTGGTTTCTCCATCACCAGTGGTGGTTACGACTGGAGCTGGATCCG17380.10970892417080758No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCACTGGGGATATTGTGATGACTCAGTCTCCCCTCTCC17310.109267058538359No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17300.10920393487658062No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCCTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17200.10857269825879691No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17050.10762584333212136No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17010.10737334868500788No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17010.10737334868500788No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16790.10598462812588373No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCACTGGGGATATTGTGATGACTCAGTCTCCCCT16570.10459590756675959No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16510.10421716559608936No Hit
GTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCCTCCGGGGTCC16250.10257595038985173No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16110.10169221912495455No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15920.10049286955116551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG306.237678E-446.11096235
GGTATCA11350.041.997881
GTGGTAT3750.040.5289651
GTATCAA24200.039.3947071
TGGTATC4400.034.5373732
TATCAAC27900.034.0422172
ATCAACG28950.032.807523
TCAACGC29400.032.3063854
CAACGCA30150.031.5037365
AACGCAG30650.030.9888366
CGATTGT850.005540585629.55069470
AGAGTAC34100.027.75056311
TATACGA500.007633580427.67532536
CGCAGAG34600.027.4510928
CAGAGTA34900.027.21512210
CGTACTT950.00953536426.44009470
ACGCAGA36050.026.3461237
TCGATTG800.001086193825.52689769
TACCTTA700.001325679424.6695527
GTAGTTT3101.70985E-1024.30782970
Produced by FastQC (version 0.11.5)