FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584192
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48500.30614975962509594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33240.2098230517513029No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG31950.20168009938189313No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAATGTAAAAT31920.20149072839655802No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30100.1900022219528946No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG29380.18545731830485196No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29130.18387922676039267No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29090.1836267321132792No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28960.1828061245101604No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28440.17952369409768512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27280.17220134933139417No Hit
CCCCTGGAGCGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG26070.16456338625621136No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC25990.1640583969619844No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25930.16367965499131418No Hit
CACCTGCACTGTGTCTGGTTTCTCCATCACCAGTGGTGGTTACGACTGGA24960.15755665979881225No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24440.154274229386337No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGAA23890.15080242798852664No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23850.15054993334141314No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22850.14423756716357614No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22660.1430382175897871No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21900.13824081929463095No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21430.13527400719104754No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21100.13319092635236132No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAATGTAAAATCTGTGCCTGATCCACTGCCA20930.13211782410212902No Hit
GGCCAAAAGTGTACGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA20790.13123409283723184No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19500.12309114046782207No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCC18970.11974558639356846No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18890.1192405970993415No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGAAAATCAGCAGAG18650.1177256292166606No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18540.11703126893709853No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACTCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18280.1153900537308609No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18010.1136857148628449No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17780.11223387064194239No Hit
GTGTCTGGTTTCTCCATCACCAGTGGTGGTTACGACTGGAGCTGGATCCG17730.11191825233305056No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCCTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17540.1107189027592615No Hit
GGCCTGGAGTGGATTGGGTTCATCTATTATACTGGGAGCACCTACTACAA17470.11027703712681292No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17390.10977204783258596No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17100.1079414616410132No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16940.10693148305255928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16360.10327031066941382No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16300.1028915686987436No Hit
CTCCCTGCCCGTCACCCCTGGAGCGCCGGCCTCCATCTCCTGCAGGTCTA16290.10282844503696521No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16000.10099785884539249No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCACTGGGGATATTGTGATGACTCAGTCTCCCCTCTCC15960.10074536419827901No Hit
GCTAATGCTCTGGGTCTCTGGATCCACTGGGGATATTGTGATGACTCAGTCTCCCCTCTCCCTGCCCGTCACCC15960.10074536419827901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)