FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428239
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.044.8900681
GTATCAA40100.041.519541
ATCAACG47250.035.0849383
TCAACGC47250.035.012154
CAACGCA47350.035.0108455
TATCAAC47600.034.7547072
AACGCAG47700.034.6818476
ACGGTAT1459.287942E-833.4560570
ACGCAGA50050.032.984717
GTGGTAT5150.031.3936771
CAGAGTA51450.031.35183310
AGAGTAC51600.031.26069611
CGCAGAG53500.030.9219478
CTCGTAT450.004580510330.72468239
GCAGAGT55800.029.6473859
TGGTATC5650.028.0017322
AGTACGG48400.027.92689713
TAACCGA500.00764610327.6658440
GAGTACG48350.027.45783812
GCGTATA809.766665E-525.7950742
Produced by FastQC (version 0.11.5)