FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365589
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2300.037.6335940
GTATCAA34400.037.0201341
GGTATCA15700.036.830221
CGTATGC2800.032.16880441
ATCAACG39850.031.9489633
TATCAAC40900.031.0446242
TCAACGC41050.031.015014
GTGGTAT7550.030.5437621
CAACGCA41950.030.4316335
CTCGTAT2750.030.194139
TATGCCG3150.029.72716343
AACGCAG43350.029.4488376
ACGCAGA46300.027.568477
CGAATTA1005.1381903E-727.52589815
TGGTATC8350.027.1982422
CGCAGAG48000.026.5920878
AGAGTAC47800.026.27245111
CAGAGTA47950.026.26105910
TATACAC4450.024.7441943
GCAGAGT52600.024.3319539
Produced by FastQC (version 0.11.5)