FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523986
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15150.289129862248228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14250.037.916161
GGTATCA6600.035.4572071
TATCAAC15250.035.1772842
GCTATAC701.0002914E-634.3749163
ATCAACG16300.033.109583
CAACGCA16650.032.80775
TCAACGC16600.032.6995624
AACGCAG17000.032.1322486
AGAGTAC17700.030.87326611
CAGTTCG1602.1890264E-730.32927770
CGCAGAG18100.030.1794598
ACGCAGA18300.029.849637
CAGAGTA18400.029.68740510
GCAGAGT18750.029.3164679
AGTACGG12500.029.14714613
GAGTACG13100.027.8121612
TCGTAAA500.007684111627.63450237
TACGGGG5700.027.13550215
TACGGGA5000.026.80987515
GTACGGG13700.026.5941114
Produced by FastQC (version 0.11.5)