FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420012
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18140.1277453993346535No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17780.12521020949118739No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16140.11366101131539733No Hit
GTATGATATTCTCCCTCCGGGATTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15920.11211172863327915No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15750.11091455565164238No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14630.10302729836085892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14600.10281603254057009No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14520.10225265701979984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25400.038.8030551
CGTATGC1100.037.7805941
TATCAAC30050.032.6804542
GTGGTAT4300.032.0571671
TCAACGC31150.031.7487664
ATCAACG31000.031.6789593
CAACGCA31800.031.0998155
AACGCAG32400.030.8429556
AGAGTAC35550.028.01014511
CGCAGAG36450.027.414038
CAGAGTA36500.027.28207610
TCGTATG1404.5292836E-1027.18792740
ACGCAGA37100.026.9337317
GCAGAGT37250.026.9177749
CGCGTAT851.4562633E-424.3214514
GCGTAGA9400.024.22130870
AGTACGG32350.023.96433313
GGTATCA18450.023.9082161
CTCGTAT1602.1409505E-923.77682339
GAGTACG32850.023.7044712
Produced by FastQC (version 0.11.5)