FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420012
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19000.13380168618293367No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17110.12049193950473658No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16820.11844970324194444No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15590.10978780461010189No Hit
GTATGATATTCTCCCTCCGGGATTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15370.10823852192798371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA200.00743705751.6127663
GTCGATT704.0138704E-544.7610570
GTGGTAT5200.037.1221
GTATCAA29400.035.9946371
TATCAAC32800.031.606162
ATCAACG33950.030.3038143
TCAACGC34100.030.1630744
CAACGCA34600.029.7261435
AACGCAG35500.029.1663196
AGAGTAC39900.026.0380311
CAGAGTA40200.025.84189810
CGCGTAT809.737571E-525.80638312
CGCAGAG40500.025.6504768
ACGCAGA41150.025.412497
GCAGAGT41950.024.7638669
GGTATCA21450.024.1051981
AGTACGG35850.022.65266413
GAGTACG36350.022.43415812
AAACGGG1500.00348472920.88848970
CGAACTA2352.1827873E-1120.53122729
Produced by FastQC (version 0.11.5)