FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457016
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31650.038.643311
GGTATCA14900.036.868521
TCAACGC37100.032.4942784
ATCAACG37800.031.9839133
CAACGCA38000.031.7246765
TATCAAC38800.031.4266682
AACGCAG39100.030.9205116
GTGGTAT4750.029.8218821
ACGCAGA41150.029.462047
TAGACCG500.007689114727.6341025
CGCAGAG43550.027.600488
AGAGTAC43550.027.44091411
CAGAGTA45200.026.59202610
GCAGAGT48100.025.0605729
GTATTAG5750.023.4337371
AGTACGG37750.021.8671213
GAGTACG38300.021.73346112
TGGTATC6750.021.4931892
TTAACGG3950.021.02111235
AGTACTT17050.020.86524413
Produced by FastQC (version 0.11.5)