FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927410
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50980.5497029361339645No Hit
GTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGCCCTGGGA39670.4277504016562254No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33220.35820187403629467No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCT32870.3544279229251356No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32120.3463408848297948No Hit
GAATTTACCCTCACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTT30400.3277946107978133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30070.3242363140358633No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCA28800.31054226286108627No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26970.29080988990845474No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCCCAGGGCCGAAAGTG26920.290270754035432No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26370.2843402594321821No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26190.28239937028930034No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24950.26902880063833684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24400.26309830603508694No Hit
GGTTATAGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCT24030.25910870057471885No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22910.24703205701900993No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA21680.23376931454265104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21520.23204407974897834No Hit
ATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGC20320.2191048187964331No Hit
CACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTT19910.2146839046376468No Hit
TCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCCTGGAAG19830.2138212872408104No Hit
GTGTAAGCTATAACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGATTT18910.2039011871771924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18620.20077419911366062No Hit
CCCCAGAGACGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGGCCGA18170.1959219762564562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCC18090.1950593588596198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17910.19311846971673802No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17460.18826624685953355No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16980.18309054247851544No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16910.18233575225628365No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCC16370.17651308482763828No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16290.1756504674308019No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15930.17176868914503832No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15690.16918083695452926No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAATCACCATCACCT15600.16821039238308838No Hit
ACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAA15290.16486774997034753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15280.16475992279574297No Hit
CTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGGCTGCACCA15190.1637894782243021No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15090.16271120647825665No Hit
CTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAG14860.16023118146235213No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14810.15969204558932942No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14650.15796681079565672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14370.1549476499067295No Hit
GGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCA13880.14966411835110685No Hit
CTCCAGGGGTGAAGTTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAATCACCATCAC13470.14524320419232054No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13460.145135377017716No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13390.14438058679548418No Hit
ATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCG13120.1414692530811615No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTTTCTGCTGCTCTGGGTTCCAG13120.1414692530811615No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13070.1409301172081388No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTT12680.1367248573985616No Hit
GAGTACTGATTTTCCATACACTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGGCTGCACCAT12350.13316656063661164No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11910.1284221649540117No Hit
GAGCATTGGTTATAGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTC11870.12799085625559353No Hit
GTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCA11650.12561865841429357No Hit
TCCCAGTCCGTCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG11350.12238384317615726No Hit
GGGTTCCAGCCTCCAGGGGTGAAGTTGTGCTGACTCAGTCTCCAGACTTT11300.12184470730313454No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11270.12152122577932091No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11140.12011947250946182No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10820.11666900292211643No Hit
GCTCTGGGTTCCAGCCTCCAGGGGTGAAGTTGTGCTGACTCAGTCTCCAG10750.11591421269988463No Hit
GCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCTCTGGGGTCCCCTCGAGGTT10700.1153750768268619No Hit
GTGTATGGAAAATCAGTACTCTGATGACAGTAATACGTTGCAGCATCTTC10470.1128950518109574No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10450.11267939746174832No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10420.11235591593793469No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10400.1121402615887256No Hit
TCCATACACTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGG10380.11192460723951651No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10330.11138547136649378No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10310.11116981701728469No Hit
AGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTTTCTGCTGCT10250.11052285396965743No Hit
GTCCGTCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGC10180.10976806374742563No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10010.10793500177914837No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9710.10470018654101206No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9650.1040532234933848No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9610.10362191479496663No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9510.1025436430489212No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9450.10189668000129393No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9390.10124971695366666No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9370.10103406260445758No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9350.10081840825524849No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9310.10038709955683031No Hit
GGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCTCTGGGGTCCCCT9290.10017144520762122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.045.7936671
GTATCAA24250.042.6121251
TATCAAC28000.036.7722132
ATCAACG28800.035.750763
TCAACGC28900.035.5079044
CAACGCA29400.034.9059075
CTCGTAT400.002561641434.59405539
AACGCAG29850.034.264326
GTGGTAT2100.032.8045731
ACGCAGA31700.032.1542977
CGCAGAG32050.031.8031628
ACCGATT800.00492732630.26611370
GCAGAGT33800.030.156559
ACGCTTA953.2188473E-729.03590228
CGTATGC605.2969076E-428.86434441
AGTACGG28650.028.60611713
GAGTACG28750.028.38684512
TGTTAGA7850.027.76000470
CGCTTAA1005.0412564E-727.58410529
AGATAGC500.007805091327.548418
Produced by FastQC (version 0.11.5)