FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927410
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52630.5674944199437143No Hit
GTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGCCCTGGGA36760.39637269384630314No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31720.34202779784561305No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31370.338253846734454No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCT31060.3349112043217132No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30750.3315685619089723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29960.32305021511521337No Hit
GAATTTACCCTCACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTT28540.3077387563213681No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27590.29749517473393644No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCCCAGGGCCGAAAGTG27410.2955542855910547No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCA25510.2750671224161913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25280.27258709740028686No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24350.2625591701620642No Hit
GGTTATAGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCT23580.25425647771751436No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22990.24789467441584628No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA22240.23980763632050547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19900.21457607746304225No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19850.21403694159001949No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19440.2096160274312332No Hit
TCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCCTGGAAG19140.2063812121930969No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18610.20066637193905607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCC18300.19732372952631522No Hit
ATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGC18300.19732372952631522No Hit
CACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTT18060.1947358773358062No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17440.18805059251032444No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17180.18524708597060632No Hit
GTGTAAGCTATAACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGATTT17140.18481577727218812No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16700.18007138158958821No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15980.17230782501806105No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15960.17209217066885196No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCC15790.17025910870057473No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15390.16594602171639297No Hit
CTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGGCTGCACCA15360.16562254019257933No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14950.16120162603379304No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14740.15893725536709763No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAATCACCATCACCT14640.1578589836210522No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14230.15343806946226587No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14130.15235979771622044No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14040.15138935314477953No Hit
ACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAA13810.14890932812887506No Hit
CTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAG13790.14869367377966597No Hit
GGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCA13570.146321475938366No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13220.14254752482720695No Hit
CCCCAGAGACGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGGCCGA13090.1411457715573479No Hit
CTCCAGGGGTGAAGTTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAATCACCATCAC13080.14103794438274334No Hit
GTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAAT12960.1397440182874888No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12820.1382344378430252No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTTTCTGCTGCTCTGGGTTCCAG12590.1357544128271207No Hit
ATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCG12060.13003957257307985No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11840.12766737473177991No Hit
GAGCATTGGTTATAGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTC11800.12723606603336174No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11730.12648127581112992No Hit
GGGTTCCAGCCTCCAGGGGTGAAGTTGTGCTGACTCAGTCTCCAGACTTT11700.12615779428731627No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11420.12313863339838906No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11410.12303080622378451No Hit
GTCCGTCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGC11400.12292297904917997No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11390.12281515187457542No Hit
GAGTACTGATTTTCCATACACTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGGCTGCACCAT11130.12001164533485728No Hit
GTGTATGGAAAATCAGTACTCTGATGACAGTAATACGTTGCAGCATCTTC11010.11871771923960277No Hit
GCTCTGGGTTCCAGCCTCCAGGGGTGAAGTTGTGCTGACTCAGTCTCCAG10930.11785510184276643No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10920.11774727466816186No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10830.11677683009672098No Hit
GGGTTTCTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAGTTGTGCTGAC10780.11623769422369826No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTT10760.11602203987448917No Hit
TCCCAGTCCGTCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG10310.11116981701728469No Hit
GTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCA10250.11052285396965743No Hit
CTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAGTTGTGCTGACTCAGTC10190.10987589092203018No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10080.1086897920013802No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9920.10696455720770749No Hit
GGGTGAAGTTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAA9900.1067489028584984No Hit
AGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTTTCTGCTGCT9820.10588628546166205No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9760.10523932241403477No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9710.10470018654101206No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9700.10459235936640751No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9690.10448453219180298No Hit
GCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCTCTGGGGTCCCCTCGAGGTT9570.10319060609654845No Hit
CTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCTCTGGGGTCCCC9550.10297495174733937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG200.00737344551.7235932
GGTATCA11200.043.6620671
TGGTATC4200.041.783332
GTGGTAT4250.040.5149271
GTATCAA27200.038.7423971
TATCAAC30950.033.90952
ATCAACG31350.033.118963
TCAACGC31350.032.9986154
CCGTTAA1056.657501E-1032.7535616
CAACGCA31900.032.4296725
AACGCAG32500.032.0424736
ACGGGTA652.3716791E-531.74575816
CGTTAAA1101.0932126E-931.26813517
ACGCAGA33950.030.7751777
CGCAGAG34500.030.2845578
GCAGAGT36200.028.958869
GAATCGA500.00692317528.2334564
AGTACGG29450.028.13752413
GAGTACG29600.028.10654412
CGAAATT1554.7293724E-1126.95116848
Produced by FastQC (version 0.11.5)