FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467734
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50080.34120624036780506No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38410.26169592037794315No Hit
GTAATACACAGCCGTGTCTGCGACGGTCACAGAGGTCAGGTTGAGGGAGA36340.2475925474234432No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34430.23457929025286597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33560.22865178567778632No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32050.21836381796701584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31070.2116868587904893No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30070.2048736351409724No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29370.2001043785863106No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29020.19771975030897967No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28940.19717469241701835No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCCTGGCCAAAAGTG27600.18804497272666573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26010.1772119471239339No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25340.17264708727875758No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24960.17005806229194118No Hit
GTGTATTACTGTGTAAGAAGTCCAGAGATGGCTACAGTCTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCCGC24950.16998993005544602No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24940.16992179781895084No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24490.16685584717666824No Hit
GTCTCTGGTGTCTCCATCAGCATGAATAGTTACTACTGGGGCTGGATCCG24460.16665145046718274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22970.1564997472294026No Hit
GTCTGCATCTGTGGGAGCGCGAGTCACCATCACTTGCCGGGCGAGTCACG22840.15561402815496542No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGCGCGAGTCACCATCACTTGCCGGGCGAGTCACGACATTAGCAA22670.15445578013454755No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22570.15377445776959586No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22450.15295687093165383No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22420.1527524742221683No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT22230.1514579617287601No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22230.1514579617287601No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21630.14737002753904999No Hit
CCATATACCTAGACACGTCCAAGAACCAGTTCTCCCTCAACCTGACCTCT21090.14369088676831088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20220.1377633821932312No Hit
GATTTTACTCTCACCATCAGCGGCCTGCAGCCTGAAGATACTGGAACATATTACTGTCAACAGTCAGATCATCTC20080.13680953088229883No Hit
GAGTAAAATCTGTCCCAGATCTACTTCCACTGAACCTTGATGGGACCCCT20040.13653700193631818No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20000.13626447299033748No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC19830.1351062249699196No Hit
ACCTACTACAACGCATCCCTCAAGAGTCGACTCACCATATACCTAGACACGTCCAAGAACCAGTTCTCCCTCAAC19290.1314270841991805No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18230.12420506713069263No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18090.12325121581976027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17890.12188857108985689No Hit
GTAGTAGGTGGTCCCAAGATAATAGATACTCCCAACCCATTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC17800.12127538096140036No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17770.12107098425191486No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17710.12066219083294386No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17560.11964020728551632No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17370.1183456947921081No Hit
GATCTACGATGCATACGAATTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTAGATCTGGGACAGATTTTA17260.11759624019066126No Hit
CTCCTGATCTACGATGCATACGAATTGGAAACAGGGGTCCCATCAAGGTT17220.11732371124468056No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCC17160.11691491782570956No Hit
CAGTAATATGTTCCAGTATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTAAAATCTGTCCCAGATCTACTTCCA17150.1168467855892144No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16900.11514347967683518No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16880.11500721520384485No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16710.11384896718342698No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16440.11200939679805741No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16410.1118050000885719No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATACTGGAACATATTACTGTCAACAGTCAGATCATCTCCCTCCCTTCA16350.1113962066696009No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16330.11125994219661056No Hit
ATATTACTGTCAACAGTCAGATCATCTCCCTCCCTTCACTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAA16280.11091928101413472No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16270.11085114877763955No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16150.11003356193969752No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGTCTCCATCAGCATGAATAGTTACT15800.10764893366236661No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15440.10519617314854053No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15400.10492364420255987No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15330.10444671854709368No Hit
GGGTAGGGGCTTTCCCAGGTTTCTGGTGATACCAATTTAAATAGTTGCTA15320.10437858631059851No Hit
GATCATCTCCCTCCCTTCACTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT15130.1030840738171903No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15070.1026752803982193No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14970.1019939580332676No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14970.1019939580332676No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14950.10185769356027728No Hit
GCCCCTACCCTCCTGATCTACGATGCATACGAATTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTAGAT14930.10172142908728694No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT14700.10015438764789805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)