FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467734
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48880.3330303719883848No Hit
GTAATACACAGCCGTGTCTGCGACGGTCACAGAGGTCAGGTTGAGGGAGA33990.23158147184707856No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33400.2275616698938636No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33150.22585836398148437No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32560.22183856202826943No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG31570.21509347061524775No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31190.21250444562843132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30780.20971102393212937No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30310.20650880881685646No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29480.20085383318775746No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28770.1960164443966005No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCCTGGCCAAAAGTG27030.1841614352464411No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26070.17762074054290491No Hit
GTCTGCATCTGTGGGAGCGCGAGTCACCATCACTTGCCGGGCGAGTCACG25870.17625809581300153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25260.17210202938679625No Hit
GTGTATTACTGTGTAAGAAGTCCAGAGATGGCTACAGTCTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCCGC25050.1706712524203977No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24850.16930860769049433No Hit
GTCTCTGGTGTCTCCATCAGCATGAATAGTTACTACTGGGGCTGGATCCG24740.16855915308904748No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGCGCGAGTCACCATCACTTGCCGGGCGAGTCACGACATTAGCAA24470.16671958270367793No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24320.16569759915625037No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23850.16249538404097744No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23140.15765799524982047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21840.14880080450544855No Hit
GATTTTACTCTCACCATCAGCGGCCTGCAGCCTGAAGATACTGGAACATATTACTGTCAACAGTCAGATCATCTC21200.14444034136975775No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20820.14185131638294132No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20340.13858096903117323No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19900.13558315062538578No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19820.13503809273342446No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT19710.13428863813197758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19670.1340161091859969No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19650.13387984471300657No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19570.13333478682104521No Hit
ACCTACTACAACGCATCCCTCAAGAGTCGACTCACCATATACCTAGACACGTCCAAGAACCAGTTCTCCCTCAAC18420.12549957962410083No Hit
GAGTAAAATCTGTCCCAGATCTACTTCCACTGAACCTTGATGGGACCCCT18200.12400067042120712No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC17940.12222923227233273No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17900.12195670332635206No Hit
GTAGTAGGTGGTCCCAAGATAATAGATACTCCCAACCCATTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC17830.12147977767088589No Hit
CTCCTGATCTACGATGCATACGAATTGGAAACAGGGGTCCCATCAAGGTT17540.11950394281252598No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17270.11766437242715641No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17250.11752810795416609No Hit
GATCTACGATGCATACGAATTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTAGATCTGGGACAGATTTTA17110.11657425664323372No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16740.11405336389291247No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16710.11384896718342698No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16670.11357643823744629No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16670.11357643823744629No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16580.11296324810898978No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16530.11262258692651393No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16270.11085114877763955No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16210.11044235535866853No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16170.11016982641268785No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16140.10996542970320236No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATACTGGAACATATTACTGTCAACAGTCAGATCATCTCCCTCCCTTCA16110.10976103299371684No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15990.10894344615577482No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15820.10778519813535695No Hit
CCATATACCTAGACACGTCCAAGAACCAGTTCTCCCTCAACCTGACCTCT15730.10717200800690044No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15570.10608189222297774No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15550.1059456277499874No Hit
GCCCCTACCCTCCTGATCTACGATGCATACGAATTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTAGAT15540.10587749551349222No Hit
ATATTACTGTCAACAGTCAGATCATCTCCCTCCCTTCACTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAA15500.10560496656751156No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGTCTCCATCAGCATGAATAGTTACT15470.10540056985802605No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT15460.10533243762153087No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15460.10533243762153087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCC15400.10492364420255987No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15290.104174189601113No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15110.10294780934419998No Hit
GGGTAGGGGCTTTCCCAGGTTTCTGGTGATACCAATTTAAATAGTTGCTA15040.10247088368873379No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15020.10233461921574345No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15000.10219835474275313No Hit
CAGTAATATGTTCCAGTATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTAAAATCTGTCCCAGATCTACTTCCA14920.10165329685079176No Hit
GATCATCTCCCTCCCTTCACTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT14850.10117637119532559No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14780.1006994455398594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.048.3551
GTATCAA29300.038.9005431
AAATCGA701.0400536E-535.8100869
TAGGTAT11450.034.5684570
TATCAAC34250.033.0581632
TCAACGC35400.031.943924
ATCAACG35500.031.8723963
AACGCAG36550.031.1269236
ACTAGAT450.00470920330.5511999
CTGGAAT17250.028.86683870
CGCAGAG39500.028.7142588
AGAGTAC40000.028.44416211
CAGAGTA40450.027.95586610
ACCGATT500.007291029727.93673954
GCAGAGT41000.027.9152269
TGTTAGA9850.027.86929370
ACGCAGA42500.026.7691527
ATCCGAC851.2918512E-424.75460259
TTCGGGG12700.024.6312370
TACGCAT851.3575557E-424.5743649
Produced by FastQC (version 0.11.5)