FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298346
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8050.043.2276731
GGTATCA3300.038.629951
TATCAAC9800.035.8600162
TACTCTA501.8127494E-434.64537440
ATCAACG10300.034.453743
TCAACGC10450.033.9591874
CAACGCA10650.033.3214535
AACGCAG11150.031.8272196
TAACGCA450.004289371431.13170858
ACGCAGA11850.029.947137
CAGAGTA11550.029.83007610
AGAGTAC11600.029.70149811
GCATTAC703.8769023E-529.5317751
CGCAGAG12200.029.087998
CCGCAAT605.281071E-428.87114540
GCAGAGT12850.027.8847379
GTGTATA1005.055699E-727.5629881
CTACACT500.007776643627.5629884
ACTATAC658.709988E-426.5028763
GAGTACG11450.026.17882212
Produced by FastQC (version 0.11.5)