FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298346
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4120.1380946954207531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA451.8986866E-645.8509916
CGCGGTA503.6648744E-641.6711543
CGAACTA503.8746766E-641.33520529
GTATCAA7850.040.7949331
GTGGTAT1500.036.7300641
ATCAACG8850.035.371533
TATCAAC8900.035.208242
TCAACGC8900.035.155124
TAACCGT601.2309434E-534.92702553
CAACGCA9100.034.382485
AACGCAG9250.033.4532246
GGTATCA3200.033.358361
GGTACCC652.1916687E-532.1036546
TAACGGC652.2980346E-531.88198336
AGTAGAC553.3072248E-431.272533
ACGCAGA9950.031.0997317
AGAGTAC9900.030.90950411
CAGAGTA10250.030.18443710
CGCAGAG10350.029.897818
CGGTACC703.6402256E-529.8054845
Produced by FastQC (version 0.11.5)