FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712675
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT7260.10186971621005368No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC7130.10004560283439155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22550.041.2085761
GGTATCA9300.038.1175231
CGCACGT650.001891127736.79630370
ATCAACG27250.033.965213
TCAACGC27700.033.5376434
CAACGCA27600.033.534495
TATCAAC28150.033.001512
AACGCAG28050.032.9965026
ACGCAGA29100.031.8036797
CGCAGAG30250.030.5946148
AGAGTAC30000.030.04679111
CAGAGTA30800.029.4897610
GCAGAGT31650.029.1325919
ACGGGTA1900.028.97239716
GAGTACG24050.027.89572112
AGTACGG24650.027.21671713
TACGGGT5000.025.45949415
GTACGGG28450.023.46051414
GTTAGCC750.002023751122.9380913
TACGGGG11250.021.71320315
Produced by FastQC (version 0.11.5)