FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396682
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34400.041.0292321
GGTATCA14100.036.4666331
TATCAAC40800.034.4043772
TCAACGC41450.034.0032544
ATCAACG41650.033.7669073
CAACGCA41650.033.7572365
AACGCAG42850.032.893476
ACGCAGA44200.031.888817
CGCAGAG45800.030.774798
TGGTATC6900.030.4901942
GTGGTAT6700.030.3882541
AGAGTAC45950.030.14825811
CAGAGTA47200.029.4228510
GCAGAGT50400.028.033359
CGCGTAT500.007778831327.56840711
GCGCATT658.7161263E-426.50808511
AGTACGG42350.023.44064913
GAGTACT17200.023.04375612
CGAACTA2400.023.0049729
GAGTACG43050.022.97696312
Produced by FastQC (version 0.11.5)