FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752990
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43790.24980176726621373No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA41370.23599678263994658No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG37070.21146726450236453No Hit
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30500.17398844260377982No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29480.1681698127199813No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29320.16725708646369916No Hit
GGCTAACACTTTCCCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT28710.16377731761162356No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28510.16263640979127092No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28020.1598411856314069No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27540.15710300686256054No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27020.15413664652964362No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26440.15082801385062095No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26330.15020051454942698No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25090.14312688606324053No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT25050.14289870449917No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24670.14073097964049994No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG24340.13884848173691808No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC24220.13816393704470648No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24200.1380498462626712No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23930.13650962070519512No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATCTTAACACCTGGTTAGCCTGGTATCAGCAGAAA23480.13394257810940166No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGA23480.13394257810940166No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23450.13377144193634877No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23450.13377144193634877No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22540.12858031135374418No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21660.12356031694419249No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20960.11956713957295821No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20650.11779873245141158No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACACTTTC19600.11180896639456014No Hit
CTGCTGCATCCAGCGTGCAAGGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA19080.10884260606164324No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19060.108728515279608No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18970.10821510676044929No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18600.10610442729279687No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18520.10564806416465582No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT18000.10268170383173891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.039.8383751
GTATCAA31700.037.387751
TATCAAC37000.031.5535582
TCAACGC38250.030.3409234
ATCAACG38600.030.0658133
CAACGCA39250.029.4799065
AACGCAG39750.029.109096
TTAACGG1800.026.89504835
AGAGTAC42750.026.82243311
CGCAGAG43450.026.6287788
CAGAGTA43650.026.26939410
TTTAACG1850.026.16292434
ACGCAGA44900.025.7688277
GCAGAGT45750.025.2145719
TGTTAGA6900.024.63177170
TCGTATG851.4112293E-424.43476940
TTCGGGG16900.023.46576970
GCGTAAG902.104689E-423.0357931
TAACGGC1854.6929927E-1022.4317736
GACGTGT13550.021.92222662
Produced by FastQC (version 0.11.5)