FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752990
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42950.2450099544207326No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA39280.22407429591726138No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG37430.2135208985789993No Hit
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC33690.19218592233840467No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32890.18762229105699404No Hit
GGCTAACACTTTCCCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT28800.16429072613078227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28470.16240822822720039No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26030.148489152818898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25910.14780460812668642No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25700.1466066549153161No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25370.14472415701173424No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG25300.14432483927461082No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25280.14421074849257554No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC25220.14386847614646975No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24840.14170075128779971No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24250.13833507321775937No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22890.13057690003936132No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22640.1291507652639205No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGA22620.12903667448188524No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22520.1284662205717089No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATCTTAACACCTGGTTAGCCTGGTATCAGCAGAAA22470.12818099361662075No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22450.1280669028345855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22420.12789576666153257No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT21850.12464417937352752No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21540.1228757722519809No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20870.1190537310537995No Hit
CTGCTGCATCCAGCGTGCAAGGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA20790.11859736792565845No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19950.11380555508017728No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACACTTTC18490.10547692799160292No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18330.10456420173532079No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18060.1030239761778447No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18020.10279579461377418No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17700.10097034210120993No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17580.10028579740899835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30950.040.213891
GTGGTAT4750.037.8480951
TATCAAC35250.035.2781262
ATCAACG35550.034.6671333
TCAACGC36700.033.4743544
CAACGCA36950.033.2478685
AACGCAG37900.032.5055276
TCGTATA450.004363070231.03201161
CGCAGAG40950.029.8316758
AGAGTAC41200.029.65742311
CAGAGTA41700.029.29680410
ACGCAGA42750.028.6563247
GCAGAGT42900.028.5561289
GTGTAAC951.03413495E-525.474681
TGTTAGA6250.024.40503370
AGTACGG41250.023.68652713
GAGTACG41500.023.62568312
CGATTGT1350.001741658423.53880370
TTCGGGG16250.021.51084370
CCGCTAT1500.003211889221.18492370
Produced by FastQC (version 0.11.5)