FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169138
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.041.4234621
GTATCAA29050.041.2333371
AACGCAG34150.034.966946
TCAACGC34200.034.8149034
CAACGCA34800.034.214655
ATCAACG34700.034.212333
TATCAAC35500.033.441352
AGAGTAC36150.031.97949611
ACGCAGA38150.031.2088857
CAGAGTA37400.031.09653310
CGTTTAG800.004838490430.38006470
CGCAGAG39750.030.0394958
GCAGAGT40500.029.6536319
CGTAAAT900.0086007927.00450170
TATATCG658.6393795E-426.547825
AGTACGG35950.026.10989413
GAGTACG36250.025.89381212
GAGTACT9000.024.92332512
GTGGTAT3800.023.618641
AGTACTT9550.023.12659513
Produced by FastQC (version 0.11.5)