FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169138
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28500.042.0541651
GGTATCA12300.037.6291241
CAACGCA33450.036.0618445
ATCAACG34150.035.3407943
TCAACGC34150.035.3256764
AACGCAG34800.034.761936
TATCAAC34950.034.271062
ACGCAGA37500.032.4428837
CGCGATA553.036031E-431.74167857
AGAGTAC37050.031.6303411
CGCAGAG39100.030.850868
CAGAGTA38350.030.6453810
GCAGAGT40800.029.5641449
GTGGTAT4250.029.2575511
TAACGTA500.007770330627.5742154
GAGTACT9850.027.29660812
AACGTAG658.7089103E-426.51145
AGTACTT10400.026.18784913
GTCTAGG1251.2504097E-724.868921
AGTACGG37700.023.7759613
Produced by FastQC (version 0.11.5)