FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74883
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5910.78923120067305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCAG205.507326E-499.3041670
TGGTATC252.95077E-453.599472
TTAGGTT252.960536E-453.5636620
CCATAGG200.00821500450.283111
GATGGCG307.0395047E-444.9365630
GTGGTAT307.228296E-444.69611
GGTATCA903.6379788E-1240.971421
CGCCTTC350.001553311238.25975812
GTATCAA2300.033.522071
ATCAACG2350.031.3404393
TATCAAC2500.029.4797042
TCAACGC2500.029.4600124
AGTACTT803.4164314E-629.31220813
CAACGCA2550.028.8823685
ACGCAGA2550.028.8823687
GAGTGCC500.006396712728.657953
AACGCAG2600.028.3269356
AGAGTAC2500.028.1209211
CGCAGAG2550.027.5695328
GTCCTGC500.00887641326.79973423
Produced by FastQC (version 0.11.5)