FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440175
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.037.825031
GGTATCA5700.033.808521
TATCAAC13900.032.6792532
ATCAACG14200.031.9888483
TCAACGC14300.031.765154
TCTTATA652.3590037E-531.7651482
CAACGCA14400.031.5445585
AACGCAG14700.030.9007916
TAGTCCG450.00466974230.5990920
ACGCAGA15600.029.1180557
AGAGTAC15700.028.71340411
CAGAGTA16050.028.08725210
CGCAGAG16200.028.0396068
GCAGAGT16750.027.1189029
TATAGAC658.778147E-426.4709573
TGGTATC2400.025.8091852
GTCTTAG1101.1758857E-625.0270861
GAGTACG14450.025.00543812
AGTACGG14550.024.59706913
TACGGGC3650.023.5700315
Produced by FastQC (version 0.11.5)