FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440175
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5950.13517351053558244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11750.036.304191
GTGGTAT2900.034.401151
TGGTATC2900.034.3698542
GGTATCA5650.034.0967141
TCGGTTT800.004149827631.34057270
GTCGGTT550.00521787229.91115669
TATCAAC14300.029.5628832
ATCAACG14650.028.833653
TAGGATC605.5675E-428.6155154
TCAACGC14900.028.57714
CAACGCA14900.028.3466425
AACGCAG14950.028.2518376
CGATTTT500.0075936827.7006338
ACGCAGA16150.026.3652537
AGAGTAC15900.026.3478711
CAGAGTA16200.025.85994310
CGCAGAG16750.025.4208248
TACGGGG6550.024.12937515
GCAGAGT17600.023.9980099
AGTACGG14900.023.28444713
Produced by FastQC (version 0.11.5)