FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204350
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54310.4509486444970316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37450.3109561174077303No Hit
GGCTTACAGTTTCCCTCCGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35200.2922738406609374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34870.2895337734047411No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC34720.2882882882882883No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34700.2881222236060946No Hit
CTGTAAGCCTGTTGACAATAATAAGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33180.27550130775937226No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32860.2728442728442729No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31930.2651222651222651No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28740.23863494831236767No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28210.23423423423423423No Hit
GTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGCCTATTAACACCTGGTTGGCCTGGTATCAGCAGAAA28020.2326566197533939No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27790.23074687590816623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27290.22659525885332338No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25900.21505376344086022No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23770.1973678747872296No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT22340.18549425001037906No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCGGAGGGAAA22260.18482999128160418No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21640.17968198613359904No Hit
ATTAACACCTGGTTGGCCTGGTATCAGCAGAAACCTGGAAGAGCCCCTAA21410.1777722422883713No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21160.1756964337609499No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20890.17345456055133474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20020.16623074687590816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19260.159920288952547No Hit
GCCTATTAACACCTGGTTGGCCTGGTATCAGCAGAAACCTGGAAGAGCCCCTAAGCTCCTGATGTATGATGCATT19180.15925603022377216No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19140.15892390085938474No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18620.15460621912234815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18570.15419105741686387No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18410.15286253995931415No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17320.14381201477975672No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATCTTATTATTGTCAACAGGCTTACAGTTTC17210.14289865902769128No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16680.13849794494955786No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16560.13750155685639556No Hit
CCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTT16370.13592394237555527No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATCTTATTATTGTCAACAGGCTTACAGTTTCCCTCCGACGTT16350.13575787769336156No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC16060.1333499398015527No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGTTCAGCTTCAGGGAGAACTGATTGTTGGACGTGTCAACCGA15930.13227051936729356No Hit
GGTATCAGCAGAAACCTGGAAGAGCCCCTAAGCTCCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCAT15340.12737161124257898No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15270.126790384854901No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15240.12654128783161042No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15130.12562793207954498No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14910.12380122057541412No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14850.12330302652883299No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14480.12023082990824928No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14240.1182380537219247No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13750.11416946900817868No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGCCTATTAACAC13510.11217669282185411No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13290.11034998131772325No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13260.11010088429443268No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13090.1086893344957861No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12880.1069456553327521No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12810.1063644289450741No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12580.10445468509984639No Hit
CTCCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCATCGAGGTT12320.1022958442313281No Hit
TTATTATTGTCAACAGGCTTACAGTTTCCCTCCGACGTTCGGCCAGGGGA12120.10063519740939096No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12100.10046913272719724No Hit
ACCTGGAAGAGCCCCTAAGCTCCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCATCGAGGTTCAGCGG12100.10046913272719724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTG200.006862124452.67319560
CTACGCT901.8189894E-1242.08931428
GTATCAA26450.040.063581
GTGGTAT2700.038.2280581
ACTACGC1009.094947E-1237.8677927
TACGACG700.002170985635.7793570
TATCAAC30600.034.7367822
CGTATGC400.002550640634.62526341
TACGCTG1102.910383E-1134.44387429
CAACGCA32000.033.5395475
ATCAACG31700.033.531413
TCAACGC31700.033.531414
TGTTAGA9450.033.12902570
AACGCAG32750.032.7714656
CGCAGAG34900.030.8473178
CGAGACT1251.2914825E-1030.28416623
AGAGTAC35650.030.10062411
CAGAGTA36150.029.68429410
ACGCAGA36400.029.6718627
GCAGAGT36500.029.3996529
Produced by FastQC (version 0.11.5)