FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204350
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52220.4335948852077884No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC37590.3121185701830863No Hit
GGCTTACAGTTTCCCTCCGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT36210.30066010711172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35030.2908622908622909No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34110.28322331548137997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32970.27375762859633823No Hit
CTGTAAGCCTGTTGACAATAATAAGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC32770.2720969817744011No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30580.2539128990741894No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29000.24079378918088595No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28530.23689126914933364No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27950.23207539336571595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26520.22020176858886537No Hit
GTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGCCTATTAACACCTGGTTGGCCTGGTATCAGCAGAAA26350.2187902187902188No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24280.20160252418316935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23630.1962054220118736No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23260.1931332253912899No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCGGAGGGAAA21700.18018018018018017No Hit
ATTAACACCTGGTTGGCCTGGTATCAGCAGAAACCTGGAAGAGCCCCTAA20510.17029933158965416No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19940.16556648814713332No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19660.16324158259642133No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19310.16033545065803131No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19280.16008635363474075No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT19000.15776144808402873No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18620.15460621912234815No Hit
GCCTATTAACACCTGGTTGGCCTGGTATCAGCAGAAACCTGGAAGAGCCCCTAAGCTCCTGATGTATGATGCATT18210.15120189313737703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17560.1458047909660813No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17460.14497446755511273No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATCTTATTATTGTCAACAGGCTTACAGTTTC17230.14306472370988502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17190.14273259434549757No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17180.1426495620044007No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17120.14215136795781957No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16890.14024162411259186No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16300.13534271598787728No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16180.13434632789471498No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15920.1321874870261967No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15840.13152322829742186No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15720.13052684020425956No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGTTCAGCTTCAGGGAGAACTGATTGTTGGACGTGTCAACCGA15660.1300286461576784No Hit
CCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTT15460.12836799933574125No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATCTTATTATTGTCAACAGGCTTACAGTTTCCCTCCGACGTT15410.127952837630257No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15010.1246315439863827No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14950.12413334993980156No Hit
GGTATCAGCAGAAACCTGGAAGAGCCCCTAAGCTCCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCAT14930.12396728525760783No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14850.12330302652883299No Hit
GTCCCAGATCCACTGCCGCTGAACCTCGATGGGACCCCAGTTTGCAAACT14260.11840411840411841No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14210.11798895669863413No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTCGATGGGACCCCA14200.11790592435753726No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13880.11524888944243782No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13630.1131730809150164No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13530.11234275750404783No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13310.11051604599991696No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13250.11001785195333583No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13060.10844023747249554No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12900.10711172001494582No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC12790.1061983642628804No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGCCTATTAACAC12160.10096732677377837No Hit
CTCCTGATGTATGATGCATTCAGTTTGCAAACTGGGGTCCCATCGAGGTT12150.10088429443268153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATC250.00829509850.20548269
GTGGTAT3700.041.8674351
GTATCAA25250.040.7638661
TAGTTAG1058.920311E-636.61724570
TATCAAC29350.034.9259872
GTACTAG400.002476409334.83439350
ATCAACG29500.034.4850853
TCAACGC29400.034.476894
CAACGCA29750.034.0712855
AACGCAG30600.033.2371446
CGATTGT1351.3051358E-633.2267670
TTATACG1101.0641088E-931.35092535
CGCAGAG32800.030.7983138
TCGGCGA450.004706060530.55492814
AGAGTAC33200.030.12427111
CAGAGTA33400.029.8347910
GCAGAGT34100.029.3218969
ACGCAGA34750.029.070067
AGTACGG30250.025.56765713
GAGTACG30600.025.3854412
Produced by FastQC (version 0.11.5)