FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803083
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTGTCAGGTTACTCATCTGCAGAAACA78210.27901421399223636No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGGTTCATCCAATTTGGAG75920.27084463785053814No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74650.2663139122173692No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57100.20370427846767292No Hit
CTATGATAGTCTCCCTTTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT53600.19121802672271923No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53540.19100397669280575No Hit
TCCCTGGTCTGTGCTGGAACCAATTTAAAAACTTGCTAATGTCATGACTCGCCTGGCAAGTGATGGTGACT53320.19021912658312293No Hit
CTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCTGTC50720.18094362528687163No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50330.17955230009243395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46120.16453312299350395No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG45880.16367692287384997No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45130.16100129749993133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44960.16039482241517644No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43460.15504357166733915No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT43110.15379494649284378No Hit
GTGTATTACTGTGCGAGGGGGGGTTGGCCGATTTTTCGAGGGCCCCTCGA42990.1533668464330168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42870.1529387463731898No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG42330.15101229610396838No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCTGTCCCAGATCCACTTCCA41030.14637454545584275No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA40890.1458750953860446No Hit
GTGCTGGAACCAATTTAAAAACTTGCTAATGTCATGACTCGCCTGGCAAG40660.1450545702713762No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC40300.14377027009189525No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40170.14330649502708268No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG39450.14073789466812078No Hit
CAGTAATACACGGCCGTGTCCTCGGCTGTCAGGTTACTCATCTGCAGAAA37240.13285371856630718No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36790.13124834334195598No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36770.13117699333198482No Hit
GTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG36690.13089159329210015No Hit
GTTCCAGCACAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACGGTTCAT36640.13071321826717225No Hit
GTACTATGTGGGCTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG36070.1286797429829941No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT35870.12796624288328246No Hit
ATATTACTGTCAGCACTATGATAGTCTCCCTTTTACTTTCGGCGGAGGGA35690.12732409279354195No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35300.12593276759910427No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34250.12218689207561817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34140.12179446702077676No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG33510.11954694170668512No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC32810.11704969135769437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32780.11694266634273763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31600.11273301575443896No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31200.11130601555501567No Hit
GTCTCCATCCTCCCTCTCTGCATCTGTAGGTGACAGAGTCACCATCACTT29980.10695366494677468No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29920.1067396149168612No Hit
CTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCACGTCATCCAATAGGTGC29020.10352886446815882No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28990.10342183945320207No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT28650.10220888928369229No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28260.10081756408925459No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28240.10074621407928341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28070.10013973899452852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)