FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074351
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18860.1755478423718133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34500.034.8148841
GGTATCA15600.033.2176061
TACCGTC450.00478310330.453367
GTGGTAT7550.029.999431
TTACTCG1054.6348156E-429.52818170
ATCAACG40950.029.2888933
TATCAAC41000.029.2722532
TCAACGC40950.029.114754
AACGCAG41000.028.9956866
CAACGCA41600.028.5774755
ACGCAGA43900.027.0022097
AGAGTAC43700.026.81469311
CGCTTAT658.30141E-426.7295743
CAGAGTA43800.026.59454310
CGCAGAG44650.026.3951848
TTAACGA809.888487E-425.93986169
TGGTATC8900.025.8140872
GCAGAGT47400.024.9349379
AGTACGG39400.022.61340513
GAGTACG39550.022.4399512
Produced by FastQC (version 0.11.5)