FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208180
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.038.980861
GTATCAA33100.038.8790861
TATCAAC39950.032.3782652
TCGTATG1400.032.16964740
TCAACGC41050.031.4268364
ATCAACG41250.031.2744643
CGTATGC1450.031.0836741
CAACGCA42250.030.5342375
AACGCAG42950.030.036596
GTGGTAT5850.029.9976141
ACGCAGA46150.027.953887
CGCAGAG47800.027.1328878
TTCGCAT550.00871996426.93000468
AGAGTAC47250.026.86402711
TGGTATC6800.026.3073432
CAGAGTA48650.026.1616710
GCAGAGT50350.025.8932349
AGTACGG38600.024.50710313
GAGTACG39050.024.2246912
TACGGGG20700.022.26891315
Produced by FastQC (version 0.11.5)