FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208180
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16980.14054197222268205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34550.038.2331661
ACGGTAT750.003190859733.0768370
TATCAAC40300.032.497732
GGTATCA15500.032.4024771
TCAACGC40900.032.0771264
ATCAACG40950.031.9620293
CAACGCA41850.031.3476735
AACGCAG42850.030.7764036
GTGGTAT7450.030.0133291
ACGCAGA45400.029.1234157
CGCAGAG46450.028.3172118
AGAGTAC46300.027.88972911
CAGAGTA46950.027.57789610
TGGTATC8250.027.0827332
CGAATTA658.8760693E-426.425315
GCAGAGT50100.026.2541799
AGTACGG39600.024.11342813
GAGTACG39700.024.05169312
TACGGGG19400.021.7804415
GTACGGG46100.020.63897714
Produced by FastQC (version 0.11.5)