FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515515
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35450.2339138840592142No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25480.1681276661728851No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22360.14754060500885838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22220.1466168266232931No Hit
GAGTTACAGTACCCCTTTCACATTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCAT21290.14048029877632356No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20730.13678518523406236No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20680.13645526438207473No Hit
GTCTCTGGTGGCTCCATCAATAATTACTATTGGAACTGGATCCGTCAGCC20570.135729438507702No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20470.1350695968037268No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19180.1265576388224465No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTACTTAAATTGGTATCAGCAGAAA19080.1258977971184713No Hit
GTCTACAACCTGAAGATCTTGCGACTTACTACTGTCAACAGAGTTACAGTACCCCTTTCACATTCGGCCCTGGGA18500.12207071523541503No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18400.12141087353143981No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18020.11890347505633399No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17930.11830961752275629No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17620.11626410824043312No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16860.11124931129022148No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16800.11085340626783634No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16710.11025954873425865No Hit
CTGTAACTCTGTTGACAGTAGTAAGTCGCAAGATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC16670.10999561205266857No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAATGTG16210.10696034021438258No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15360.10135168573059324No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15230.10049389151542545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27250.036.8532521
TATCAAC32000.031.3776682
GTGGTAT4550.031.0972331
TCAACGC32750.030.448384
ATCAACG33300.030.0490953
CAACGCA33650.029.634015
TGGATTG9300.029.46744270
AACGCAG35200.028.4261916
AGAGTAC38950.025.60081111
ACGCAGA39150.025.5581597
CGCAGAG39550.025.386918
GCAGAGT40950.024.4347239
CAGAGTA42300.023.6548910
TACACCC8400.022.592335
TTCGGGG10250.022.4828170
GGTATCA26600.021.6662711
GACGTTA950.002893146121.5707969
AGTACGG32850.021.53187213
GAGTACG33450.021.248812
GTAAGCG850.003987139620.40998546
Produced by FastQC (version 0.11.5)