FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515515
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35620.23503561495597206No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24730.163178853393071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22440.14806847837203857No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22180.14635288994170298No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20120.13276015083981352No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20020.1321003091358383No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTACTTAAATTGGTATCAGCAGAAA19710.13005479985351515No Hit
GAGTTACAGTACCCCTTTCACATTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCAT19350.12767936971920438No Hit
GTCTCTGGTGGCTCCATCAATAATTACTATTGGAACTGGATCCGTCAGCC19300.12734944886721677No Hit
GTCTACAACCTGAAGATCTTGCGACTTACTACTGTCAACAGAGTTACAGTACCCCTTTCACATTCGGCCCTGGGA18270.12055307931627202No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17850.11778174415957611No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17410.11487844066208518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17170.11329482057254464No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17080.11270096303896696No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16780.11072143792704131No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16760.11058946958624626No Hit
CTGTAACTCTGTTGACAGTAGTAAGTCGCAAGATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC16710.11025954873425865No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16660.10992962788227104No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAATGTG16630.10973167537107849No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16520.10900584949670575No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16100.10623451434000983No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15950.10524475178404702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)