FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252429
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA43070.34389174955227003No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35020.27961664892780347No Hit
ATACTATTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA27250.21757720397723146No Hit
ATATTATACTATTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24890.19873382044012078No Hit
CTCCTAAACTACTCATTAACTGGGCATCTACCCGGGAATCCGGGGTCCCT24340.19434235393782803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23920.19098887042698628No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC23670.1889927492895805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22990.18356329979583672No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21040.16799355492407153No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATTCG19990.15960984614696722No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT19180.15314241366177245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18840.15042768891490058No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA18660.1489904816959684No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18660.1489904816959684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18580.14835172293199855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18540.1480323435500136No Hit
GAATAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18140.14483854973016433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17360.13861065178145826No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16440.13126492599580497No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16390.1308657017683238No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15650.12495718320160265No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15470.12351997598267048No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14790.1180905264889267No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14460.11545564658755106No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14280.1140184393686189No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14130.11282076668617541No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14040.11210216307670934No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13850.11058511101228094No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13620.1087486795658676No Hit
ATCCTAGAGTCCCCGCTCTGGCACAGAAATATACGGCCGTGTCTGCGGGA13610.10866883472037138No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13100.10459674760006354No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13040.10411767852708616No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13010.10387814399059746No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA12850.10260062646265777No Hit
AACCAGGACAGCCTCCTAAACTACTCATTAACTGGGCATCTACCCGGGAA12790.10212155738968037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12710.10148279862571051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25000.042.264771
GGTATCA9500.041.4360471
TATCAAC28000.037.598062
GTGGTAT3000.036.8320431
TCAACGC28700.036.6810344
ATCAACG29200.036.0529373
CAACGCA29750.035.73595
AACGCAG30400.034.9704176
CTACTCA20700.034.04621570
AGAGTAC32300.032.37256211
CGCAGAG33600.031.5308768
CAGAGTA33600.031.1200510
GCAGAGT34150.030.8209539
ACGCAGA34650.030.5753967
TGGTATC3700.028.9190222
GAGTACG30050.026.29726612
AGTACGG30000.026.2260713
TGTTAGA5250.026.13509870
TAGCTTG16500.025.13183432
TACTTAG16650.024.88353328
Produced by FastQC (version 0.11.5)