FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443001
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35650.040.180881
GGTATCA16400.037.4633871
TCAACGC40350.035.0684
TATCAAC41400.034.5120432
ATCAACG41000.034.5120433
CAACGCA41800.033.9329155
AACGCAG43750.032.3415956
ACGCAGA46700.030.3640847
CGCAGAG47850.029.7064328
AGAGTAC47450.029.08432811
GTGGTAT6050.028.5263061
CAGAGTA48700.028.47949810
GCAGAGT52350.027.2187779
TTAACGG2900.023.83941835
AGTACGG39050.023.7665713
GAGTACG39400.023.64301112
TAACGGC2950.023.44187236
GAGTACT20100.022.65755812
CGCGGTA3150.022.03921743
TGGTATC7650.021.6546172
Produced by FastQC (version 0.11.5)