FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274628
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44850.041.984711
CAACGCA51550.036.1845935
ATCAACG52050.035.6566663
TCAACGC52300.035.4698374
TATCAAC54150.034.6245462
AACGCAG54550.034.0053376
GGTATCA19600.033.6076621
ACGCAGA58400.031.8224757
AGAGTAC58400.031.23454311
CAGAGTA61000.029.95834210
CGCAGAG62300.029.8303788
GCAGAGT70100.026.5602579
GTGGTAT8850.025.719451
AGTACGG54800.025.43922813
GAGTACG55350.025.1847812
TACGGGG27900.022.57802215
GTGTATA4600.022.4918121
TGGTATC10150.021.7318922
GTACGGG65250.021.04853214
ACGTTAG1500.003761123920.6163570
Produced by FastQC (version 0.11.5)