FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579523
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1700.044.6616331
GTATCAA13350.042.912881
TTATACG350.001311766639.64328846
ATACCGT350.00134624739.4346356
TATCAAC14950.038.7785382
CAACGCA16500.035.5509225
TGGTATC2250.035.2751352
TCAACGC16850.034.6076974
ATCAACG16950.034.4035223
AACGCAG17450.033.6154826
GGTATCA5150.033.5060771
CGTAGGT750.003082528733.30555770
ACGCAGA18350.031.9667687
CGCAGAG18850.031.1188458
CAGAGTA18750.030.91675610
TATACGA450.0044824930.85775847
TACCGTC450.004617343730.6713837
AGAGTAC19000.030.3283511
GTGTATA1601.8189894E-1228.04041
GCAGAGT20700.028.0043059
Produced by FastQC (version 0.11.5)