FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697897
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22580.3235434455227634No Hit
GTATAATAACTGGCCCTTCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT22380.32067769312663613No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20180.2891544167692367No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCCGCTGATGGTGAGAGTGAACT15580.2232421116583106No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14040.201175818208131No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12820.18369472859175495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12800.18340815335214222No Hit
CCTCATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTT12770.18297829049272313No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATT12320.1765303476014369No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12260.17567062188259874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12160.1742377456845351No Hit
GAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATC11450.16406432467828347No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAG11420.16363446181886437No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11360.1627747361000262No Hit
GAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAACTGG11310.16205829800099442No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11090.15890597036525447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10940.15675665606815906No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10910.15632679320874No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10770.1543207665314509No Hit
CTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCACT10630.15231473985416186No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10310.14772953602035832No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10060.14414734552519928No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9960.14271446932713566No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATGAAGGGCCAGTTATTATACTG9910.14199803122810387No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9750.13970542931120208No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9640.1381292654933321No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9410.1348336502377858No Hit
GTCCCAGACCCACTGCCACCGAACCTGGCTGGGATATCAGTGGCCCTGGT9210.13196789784165858No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9190.13168132260204587No Hit
TTCCTGTACAGCCTCTGGATTTAGTTTTAGTAGCTATTGGATGAGCTGGGTCCGGCAGGCTCCAGGGAAGGGG9180.13153803498223948No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTA9010.12910214544553136No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8350.11964516253831153No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8180.1172092730016034No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8040.11520324632431433No Hit
GTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATCCA7820.11205091868857439No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7520.10775229009438356No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG7510.10760900247457719No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7490.10732242723496448No Hit
ATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAGGTTGCTGTTAATACTCTGACTGGC7380.1057462634170945No Hit
CATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCA7350.10531640055767542No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7260.10402681197941817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7080.10144763482290366No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGT7050.10101777196348458No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7000.10030133386445278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.043.189191
GTATCAA12650.043.155051
TATCAAC14550.037.2715262
ATCAACG15250.035.7872053
AACGCAG15500.034.989656
TCAACGC15500.034.9871444
CAACGCA15550.034.8771445
GTTAGAT400.002580401534.5414473
AGAGTAC16600.032.6710611
CGCAGAG16850.031.981328
ACGCAGA17350.031.2587667
TGTATAC450.004594394530.703513
GCAGAGT18050.029.8551359
CAGTTCG7850.029.2676970
AGTACTT5150.028.84043313
GTGTATA605.382098E-428.7845413
GAGCAAA4750.028.21508270
GAGTACT5300.028.02419512
AGCGCTG2750.027.635145
ATATACC756.109928E-527.633163
Produced by FastQC (version 0.11.5)