FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697897
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21860.3132267368967054No Hit
GTATAATAACTGGCCCTTCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT21120.30262345303103466No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18400.2636492204437044No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCCGCTGATGGTGAGAGTGAACT14770.21163581445399535No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13350.1912889724414921No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12540.17968267523717682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12460.17853637427872596No Hit
CCTCATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTT12330.17667363522124324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11660.16707336469421707No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATT11550.1654972008763471No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11110.1591925456048672No Hit
GAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAACTGG10930.1566133684483527No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10410.1491624122184219No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10400.14901912459861555No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10300.14758624840055193No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATGAAGGGCCAGTTATTATACTG10060.14414734552519928No Hit
GAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATC10050.14400405790539292No Hit
CTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCACT9900.1418547436082975No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAG9640.1381292654933321No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9570.13712625215468757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9490.13597995119623668No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9390.1345470749981731No Hit
TTCCTGTACAGCCTCTGGATTTAGTTTTAGTAGCTATTGGATGAGCTGGGTCCGGCAGGCTCCAGGGAAGGGG9250.13254104832088404No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9120.13067830926340132No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9110.13053502164359498No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9110.13053502164359498No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9110.13053502164359498No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9050.1296752959247568No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8970.1285289949663059No Hit
GTCCCAGACCCACTGCCACCGAACCTGGCTGGGATATCAGTGGCCCTGGT8940.12809913210688684No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTA8260.11835557396005426No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8260.11835557396005426No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8230.11792571110063518No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7710.1104747548707044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7690.1101881796310917No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7550.10818215295380265No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG7500.10746571485477083No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC7380.1057462634170945No Hit
GTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATCCA7240.10374023673980545No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7220.10345366150019272No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT7220.10345366150019272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7150.1024506481615482No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGT7120.10202078530212912No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7090.10159092244271004No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6990.10015804624464641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.040.9014851
GGTATCA5350.037.4719431
TATCAAC13850.035.4025152
GTTATAC709.706673E-734.510113
TCAACGC14500.033.534064
AACGCAG14600.033.0705456
ATCAACG14750.032.9893263
ACGCTTT450.003302798132.84473468
CAACGCA14900.032.633825
TGGTATC3300.031.3908352
GTGGTAT3300.030.3749851
AGAGTAC16100.029.7773611
CGCAGAG16600.029.2918038
GCAGAGT17000.028.3997279
ACGCAGA17100.028.2356687
TACACCC4650.027.4399815
CGCGGTA658.399273E-426.67438943
ACGAACT7500.025.52706355
AGTACGG15550.025.28913713
CGAACTG7600.025.1966556
Produced by FastQC (version 0.11.5)