FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889660
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATCGTTATCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT46390.5214351550030348No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37630.4229705730278983No Hit
CGATAATACTGTTCGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC34300.3855405435784457No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20680.23244835105545938No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20300.2281770564035699No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20200.22705303149517791No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19090.21457635501202707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18260.20524694827237372No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17980.20209967852887623No Hit
AGTCAGGACATTAGCAATTATGTAGCCTGGTTTCAGCAGAAACCTGGGAA17290.19434390666097162No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG16960.1906346244632781No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16740.18816176966481576No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGTCGGGCGAGTCAGG16380.1841152799946047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16050.18040599779691116No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT15790.17748353303509207No Hit
GATCTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA15760.17714632556257445No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT14870.16714250387788593No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT14800.16635568644201157No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGCGAACAGTATTATCGTTATCCGGTCACCTT14380.16163478182676527No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT14370.1615223793359261No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14000.1573634871748758No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13770.15477822988557427No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13340.1499449227794888No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC13110.14735966549018728No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12180.13690623384214196No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG11810.13274734168109165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11600.1303868893734685No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA11380.1279140345750062No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC11150.12532877728570466No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10870.12218150754220714No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10870.12218150754220714No Hit
GTCCCTGATCTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT10620.11937144527122721No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10230.11498774812849853No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10060.11307690578423218No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10000.11240249083919698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9920.11150327091248341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9750.10959242856821708No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA9740.10948002607737788No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGCGAACAGTATTATCGTTAT9630.10824359867814672No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9610.10801879369646832No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9560.10745678124227233No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9460.10633275633388035No Hit
GTAGGAGACAGAATCACCATCACTTGTCGGGCGAGTCAGGACATTAGCAATTATGTAGCCTGGTTTCAGCAGAAA9270.1041971090079356No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG9170.10307308409954363No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9050.10172425420947329No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCGGATAACGATAATACTGTTCGCAGTAATAAGTCGCAAAATCTTCAGG9040.1016118517186341No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9020.1013870467369557No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA8990.10104983926443811No Hit
GTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT8950.10060022930108131No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC8910.10015061933772453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT451.8779338E-645.9392628
GGTATCA7700.041.231241
GTATCAA18050.039.766321
GTGGTAT2650.036.462081
TATCAAC20450.035.2542232
TGGTATC2900.033.305672
ATCAACG22150.032.8322373
TCAACGC22300.032.6003954
CAACGCA23200.031.3357225
AACGCAG23650.030.885176
ACGCAGA25100.029.1009657
CGCAGAG25150.029.0431128
AGAGTAC25600.028.40119211
CAGAGTA26150.027.66896410
GCAGAGT26650.027.4068769
TAATCAA950.00955943226.42413770
GTATAAT1001.5600754E-524.1561261
GAGTACG21650.024.0373412
AGTACGG21650.023.8781513
GAGTACT9000.023.35902212
Produced by FastQC (version 0.11.5)