FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278939
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17470.136597601605706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.039.587131
GGTATCA17000.034.0055241
AACCGGT750.003240287332.973570
TATCAAC47850.032.5536232
ATCAACG49100.031.705013
TCAACGC49200.031.6992594
CAACGCA50200.030.9993695
AACGCAG50550.030.852696
ACGCAGA53350.029.362217
AGAGTAC53950.028.71841211
GTGGTAT7750.028.416291
CGCAGAG54850.028.3713448
CAGAGTA55650.027.84111810
GCAGAGT58850.026.6180789
CGAATTA658.858725E-426.434315
TATCCCG809.845251E-525.7643745
CGATTGT1200.001029491225.76054670
AGTACGG48200.024.6664313
GAGTACG48900.024.59056312
TGGTATC8950.024.2047482
Produced by FastQC (version 0.11.5)