FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064098
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC85760.8059408062039398No Hit
GGTATACACTGGCCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT55170.5184672840283508No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37320.3507195765803525No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA35180.3306086469479315No Hit
GTATACACTGGCCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA29670.2788277019597819No Hit
AGGTATACACTGGCCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28790.27055778697074895No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT28340.2663288531695389No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT24820.23324919321340704No Hit
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA24750.23259135906655215No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC24690.2320275012263908No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTCATTTATAAGGTTT24620.2313696670795359No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24310.22845640157203567No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22240.20900330608646947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21920.20599606427227565No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21790.20477437228525944No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20600.19359119178872622No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20280.19058394997453243No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19960.1875767081603386No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT19690.1850393478796126No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG19560.18381765589259635No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGAC18480.17366821476969227No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC17670.1660561339275142No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT17330.16286093949993327No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16720.15712838479162633No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA16550.1555307875778359No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCCGGCCGTGGCACTCATTTA16340.15355728513727118No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16010.15045606701638384No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG15960.14998618548291603No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15720.1477307541222707No Hit
GTCTGGGACCCCAGAGCCCCGGTTAGAAACCTTATAAATGAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA15380.14453555969468976No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC15280.14359579662775424No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15170.14256205725412507No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15160.14246808094743152No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG15080.14171627049388308No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT14850.13955481543993128No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA14690.13805119453283438No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC14600.13720540777259238No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTACCTATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG14600.13720540777259238No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14470.13598371578557614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14430.13560781055880192No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG14360.13494997641194703No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC14310.13448009487847926No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATAGGTAC14040.13194273459775321No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT13890.13053308999734986No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13880.1304391136906563No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC13870.13034513738396275No Hit
ATGTATTACTGTACGAGGGGGGGCAGCTGGTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC13030.12245112762170401No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA12900.12122943563468778No Hit
CCTTTAGTACCTATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGC12860.12085353040791355No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG12830.1205716014878329No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12520.11765833598033264No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA12470.11718845444686485No Hit
GGCCAGGCCAATCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGGC12430.11681254922009063No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT12240.11502699939291305No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11980.1125836154188806No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC11870.11154987604525146No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11820.11107999451178369No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11730.11023420775154169No Hit
CGTCCATACCGTACCAGCTGCCCCCCCTCGTACAGTAATACATAGCCGTG11680.1097643262180739No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11630.10929444468460613No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT11540.1084486579243641No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA11290.10609925025702521No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGG11240.10562936872355742No Hit
GTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGACCTCAGGGGT11140.10468960565662186No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCCAGATGGAAGTGAGAAATCCTA11030.10365586628299274No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10930.10271610321605718No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10920.10262212690936362No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC10790.10140043492234738No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10770.10121248230896027No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC10660.10017874293533115No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10660.10017874293533115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGTG20500.054.69323770
GTGGTAT2250.050.639061
TGGTATC2250.049.0906942
GTATCAA16650.042.5102651
GGTATCA6950.042.226811
TATCAAC19300.036.663022
AATCAGT21050.034.70970569
TCGTATG400.002557949434.60470640
ATCAACG20650.034.2661673
GTATACC18850.033.1435433
CAACGCA21250.033.136225
GCAGTAA18550.033.12133416
TCAACGC21300.033.0584344
TGCAGTA18450.032.92668515
TGTATAC18800.032.8644872
TATACCT18800.032.8644874
ATGCAGT18500.032.83769214
TACCTTG18850.032.7773176
ATACCTT18850.032.7773175
AACGCAG21550.032.6749276
Produced by FastQC (version 0.11.5)