FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691642
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.040.309241
GTATCAA45500.038.9966851
TATCAAC54800.032.1789672
TCAACGC55200.032.1316534
ATCAACG55100.032.06523
CAACGCA55900.031.9137615
AACGCAG57750.030.9509376
GTGGTAT6200.029.3950061
ACGCAGA61400.029.1092977
CGCAGAG63550.028.1244838
AGAGTAC64200.027.30435611
CAGAGTA65350.027.0342510
GCAGAGT70450.025.4187099
TGGTATC7450.025.377082
GAGTACG49500.024.71960612
AGTACGG49800.024.50167713
TCGTATG1352.5664667E-723.086240
TATGCGT750.002037176622.91424410
ATACCGC800.002962511321.4833746
TACGAAA1450.00351447320.8584870
Produced by FastQC (version 0.11.5)