FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691642
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18710.11060259794921148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52600.039.4949231
GGTATCA21250.035.9322241
GTGGTAT8900.035.553951
TCAACGC62650.032.712074
TATCAAC63800.032.54142
ATCAACG63300.032.4990353
CAACGCA63500.032.3272975
AACGCAG64400.031.928826
TGGTATC9800.031.5672282
ACGCAGA66950.030.712717
CGCAGAG70600.029.172638
AGAGTAC70000.028.89095511
CAGAGTA71000.028.62234910
GCAGAGT76350.026.975619
GAGTACG58450.025.20023512
AGTACGG58800.024.93566113
TAGACCG750.002052364222.8849875
GTACGGG68050.021.49570814
GAGTACT26150.021.40167212
GTACTTT26400.020.81068614
Produced by FastQC (version 0.11.5)