FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410572
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC4130.10059137008855937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT200.00743630851.6075110
GGTATCA5650.045.0833851
GTGGTAT1800.038.2464141
GTATCAA11400.035.327611
ATCAACG12900.031.2121433
TCAACGC13100.030.9983184
TGGTATC2000.030.9720482
AGTCGAT450.004681990530.58222610
CAACGCA13400.030.3043275
TATACAC703.9115646E-529.4971893
AACGCAG14000.029.2513796
TATCAAC14300.028.1564062
ACGCAGA14500.028.0019667
AGTACGG12000.027.81071313
TCGTATG500.007553917427.73001140
TCTCGTA500.00762658327.67567838
GGTCGCA500.0077828327.56092630
ACCGTAC500.007806019427.5441327
TCTCCGA500.00783391927.52400211
GAGTACG12350.027.30113612
Produced by FastQC (version 0.11.5)